Congratulations to Benjamin Barti! 09/30/2024 |
Congratulations to Beni on defending summa cum laude his PhD thesis: “Regulation of the tonic endocannabinoid signaling pathway in hippocampal inhibitory cells”. Beni completed his PhD studies at the János Szentágothai Neurosciences Doctoral School of the Semmelweis University. |
Welcome to Caroline! 08/12/2024 |
We are happy to welcome Caroline Shumaker from Indiana University Bloomington! Caroline will strengthen our projects with her expertise in bioinformatics. We wish Caroline a pleasant and fruitful time in Budapest! |
Our new study is highlighted on the cover of Science Advances’ May issue
05/29/2024 |
We are delighted to announce that our new study on cannabinoid signaling led by Benjamin Barti appeared on the cover of Science Advances. We developed an approach in which we measured the physiological, the anatomical and the nanoscale molecular parameters from a single synapse in the brain. This helped us to discover a new molecular principle controlling the strength of synapses and we also showed that the psychoactive substance in cannabis affects this mechanism thereby changing synaptic activity.
Congratulations to Benjamin and all co-authors!
Link to the study:
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Susanne Prokop is awarded the prestigious Junior Prima Prize
11/27/2023 |
We are really proud that Susanne’s dedication and hard work has led her to win the Junior Prima Prize in Hungarian science category!
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Dárius Leszkó wins first prize at Semmelweis University Korányi Frigyes Scientific Forum
03/18/2023 |
Congratulations to Dárius for winning first prize at the ‘Translational medicine and diagnostics’ Sections of the 27th Korányi Frigyes Scientific Forum of the Semmelweis University.
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Special issue of “Cell adhesion molecules in neural development and disease” coedited by Robert Hindges from King’s College London and Zsolt Lele
02/16/2023 |
This special issue of Frontiers in Neuroscience features the diverse roles of cell adhesion molecules spanning from the well-known adhesion and mechanical stabilization to initiation of intracellular signaling cascades or acting as extracellular signaling molecules after cleavage.
![]() Zsolt Lele together with his former PhD student, Zsófia László also authors a review in this special issue. They summarize recent advances in the field of N-cadherin research: “Flying under the radar: CDH2 (N-cadherin), an important hub molecule in neurodevelopmental and neurodegenerative diseases”. |
Szentágothai Day at the University of Pécs
11/14/2022 |
Our PI had the honour to be an invited speaker at the Szentágothai Day commemorating the 10th anniversary of the János Szentágothai Research Centre of the University of Pécs. István presented the group’s latest achievements in his talk entitled: Nanoscale integration of functional anatomy and pharmacology by super-resolution imaging.
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Our new paper in Endocannabinoid Signaling: Methods and protocols
09/25/2022 |
Miki Zöldi and István Katona give a comprehen
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A new article is published in collaboration with the lab of Ádám Dénes
09/20/2022 |
The study elucidates the pivotal role played by P2Y12 receptors on microglial processes in neurodevelopment by establishing junctions with cell bodies of developing neurons throughout embryonic, early postnatal, and adult neurogenesis. For further reading, please, refer to:
https://www.cell.com/cell-reports/fulltext/S2211-1247(22)01201-3 Graphical abstract of the paper: |
Susanne Prokop successfully defended her PhD dissertation 06/29/2022 |
Congratulations to Susanne on defending summa cum laude her PhD dissertation titled “Advanced fluorescent ligand-based techniques for the pharmacological and anatomical examination of dopamine receptors”.
Susanne completed her PhD studies at the János Szentágothai Neurosciences Doctoral School of the Semmelweis University. |
Márton Vámosi wins first prize at Semmelweis University’s Rector’s Award01/04/2022 |
Congratulations to Márton for winning the Rector’s Award!
Marci is in his final year |
Susanne Prokop receives the Institute of Experimental Medicine’s Young Investigator Award
12/17/2021 |
Congratulations to Susanne for winning the Young Investigator Award!
With this prize the Institute of Experimental Medicine rewards each year thre |
A new paper from @Katonalab in Nature Communications!
10/15/2021 |
We are delighted to share the news that our latest study was just published in the prestigious journal Nature Communications!
The study introduces PharmacoSTORM a novel method based on fluorescent small molecule pharmacoprobes (receptor ligands and enzyme inhibitors) and STORM super-resolution imaging. By using the logic of autoradiography and combining its quantitative strengths with the power of immunostaining for the visualization of cellular and subcellular profiles in intact brain circuits, PharmacoSTORM makes nanoscale pharmacological measurements possible in a cell-type- and subcellular compartment-specific manner. We demonstrated broad applicability of PharmacoSTORM for GPCRs, ion channels and enzymes. Most importantly, PharmacoSTORM revealed that the major binding sites of cariprazine, a novel antipsychotic and antidepressant are the D3 dopamine receptors located on the axons of the Islands of Calleja granule cells. The physiological role and the neuropsychiatric significance of the Islands of Calleja are not well understood. Therefore, our new study has important translational impact by postulating that a major mechanism of action of cariprazine treatment is the regulation of the dopaminergic control of the outputs of the Islands of Calleja granule cells via D3 receptor binding. The first author of the study is Susanne Prokop, an exceptional graduate student in our lab. Several other former and current lab members contributed to this work including Benjámin Barti, Márton Vámosi, Miklós Zöldi, László Barna, Gabriella Urbán and Barna Dudok. We are very grateful for the valuable contribution of our wonderful collaborators at the Keserű lab, at the van der Stelt lab, at the Hunyady lab and Gian Marco Leggio. |
The @Katonalab at the Cannabinoid Gordon Research Conference10/12/2021 |
The first in-person conference after the long period of social isolation was a tremendous success in all regards. The talks of the terrific line-up of world class speakers and the more than 100 exciting unpublished posters helped to gain new knowledge on technology and to understand the conceptual framework in several hot topics of neuroscience in Ventura, California for 4 days. Between-session discussions helped to launch four new collaborative projects with outstanding investigators of complementary expertise.István Katona, our PI received the honor from the cannabinoid community to give the Keynote Talk together with Prof. Bryan Roth, a towering figure of the GPCR field and of chemogenetic interrogation of neuronal activity on the first evening.
The @Katonalab presented three major unpublished projects. István introduced PharmacoSTORM, our new approach to perform cell-type-specific nanoscale pharmacology in complex brain circuits. Benjámin Barti presented our on-going research on tonic cannabinoid signaling and Miklós Zöldi shared his new methodology for nanoscale imaging of astrocytes. As icing on the cake, the best poster prizes of the Cannabinoid GRC was received by Benjámin Barti, and by Barna Dudok, former graduate student of the @Katonalab, who is now a postdoc at Stanford University. Congrats! |
New success at the National Student Competition
04/12/2021 |
Congratulations to Márton Vámosi and his supervisor, ![]() |
New collaborative paper published in Neurobiology of Disease: “Detrimental impacts of mixed-ion radiation on nervous system function”01/05/2021 |
In this NIH funded collaboration with the labs of Prof. Charl |
Members of our team join the new Bloomington lab01/04/2021 |
Our young colleagues, Petra Aradi, Diana Dimen, Benjamin Barti and Miklos Zoldi are excited to start their work at Istvan Katona’s new „Addiction and Neuroplasticity laboratory” at the Department of Psychological and Brain Sciences, Indiana University. |
Congratulations to Zsófia László on winning the Junior Prima Award in Science!12/10/2020 |
The Junior Prima Prize in Science was founded in 2007 by the Hungarian Development Bank. It is awarded every year to ten young scientists for their outstanding scientific achievement. We are really proud and happy that Zsofi’s pivotal work on uncovering the protective role of the endocannabinoid enzyme ABHD4 during brain development has been recognised by this prestigious prize.
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A new paper from our lab in Cerebral Cortex11/24/2020 |
For further reading please see the |
Congratulations to Zsófia László on successfully defending her PhD thesis!09/15/2020 |
Dear Zsófi, we wish you a lot of success for the forthcoming years! You have been the engine and heart of the lab and we will definitely miss you a lot! |
Success at the Semmelweis University Scientific Student Competition09/04/2020 |
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A new paper in Nature Communications identifies the protective role of the endocannabinoid enzyme ABHD4 against pathological insults during brain development
08/31/2020 |
We are delighted to announce that one of our major research projects has now been published in Nature Communications.
During a difficult, but very rewarding scientific journey, Zsófia László and Zsolt Lele, the main authors of the paper, discovered a new safeguarding mechanism that we termed developmental anoikis. This process is conceptually analogous to anoikis, a detachment-induced form of apoptosis that is the central protective mechanism against pathological forms of epithelial-mesenchymal transition. By using an arsenal of state-of-the-art methods such as in utero electroporation and correlated confocal and super-resolution imaging, and with the help of other lab members such as Miklós Zöldi, Vivien Miczán and Fruzsina Mógor, they succeded in identifying the serine hydrolase abhydrolase domain containing 4 (ABHD4) as an essential molecular player in this protective mechanism. ABHD4 is expressed in radial glia progenitor cells and triggers apoptosis upon abnormal delamination of these cells thereby preventing pathological developmental processes. Importantly, the rapid downregulation of ABHD4 was observed following neuronal cell fate commitment under normal conditions indicating that the delaminated daughter neuroblasts escape from anoikis by silencing ABHD4-mediated developmental anoikis. Maternal alcohol exposure often results in microcephaly due to the loss of radial glia progenitor cells. To test the relevance of ABHD4 in cell death induced by teratogenic insults, Zsófi and Zsolt also demonstrated the clinical importance of ABHD4-induced cell death in two mouse models of fetal alcohol syndrome. For further reading please see the homepage of Nature Communications: https://www.nature.com/articles/s41467-020-18175-4 |
A new book titled ’From Bud to Brain: A Psychiatrist’s View of Marijuana’ highlights our PI’s early contribution to cannabinoid research as one of the ’Monumental Marijuana Discoveries’ 05/27/2020 |
To resonate with the growing legalizatio He introduces the basic neurobiological effects of cannabis and the operational principles of the endogenous cannabinoid signaling system in the first chapter in a historical context called „Monumental Marijuana Discoveries”. Starting from the ground-breaking isolation of THC by Raphael Mechoulam in 1964 via the discovery of the cannabinoid receptors and their endocannabinoid messengers in the 90’s, the chapter ends with elucidation of synaptic endocannabinoid signaling. We are proud that the doctoral and postdoctoral findings of our PI achieved in the lab of Tamas Freund in Budapest are introduced in details and were selected as one of the key discoveries in cannabinoid research. We advise to order the book for further reading, but Chapter 1 is freely available here: |
A new study in collaboration with the lab of Prof. Susana Mato04/01/2020 |
Ana Bernal-Chico worked with us with a postdoctoral fellowship and just returned to her home Basque Country. An interesting study partly arising from her studies in Budapest and titled: ’P2x7 receptors control demyelination and inflammation in the cuprizone model’ has been published in Brain, Behavior, & Immunity Health. The lead author is Prof. Susana Mato in the Achucarro Basque Center for Neuroscience. The study shows that P2x7 receptors are required for the onset of demyelination and T cell-independent inflammation, but not for the subsequent myelin repair process. For further reading see: https://www.sciencedirect.com/science/article/pii/S2666354620300272 |
Preview article in Neuron by Istvan Katona03/18/2020 |
In an outstanding article in Neuron, David Marcus and his colleagues from the Sachin Patel lab identified a crucial mechanism of stress resilience that involves the retrograde action of 2-AG endocannabinoid molecule at a specific synaptic connection between BLA pyramidal neurons and the supragranular pyramidal neurons in the prelimbic prefrontal cortex (plPFC). With the aid of in vivo imaging techniques and cell-type-specific genetic manipulations they show that breakdown of this retrograde endocannabinoid signaling is the essential switch for the development of stress-induced anxiety-like behavior in mice. Our PI had the privilege to provide a context for this work in a Preview Article for the readers of Neuron. By using the famous Michael Douglas movie ’Falling down’ as an example, the preview article also aims to highlight how important it is to better understand the mechanisms of stress resilience together with the underlying neurobiological processes that make some individuals more vulnerable to stressful conditions leading to mental illnesses and breakdown. For further reading see: https://www.cell.com/neuron/fulltext/S0896-6273(20)30149-5 |
A new publication from our lab in Cerebral Cortex03/14/2020 |
Our new paper titled ’N-cadherin (Cdh2) Maintains Migration and Postmitotic Survival of Cortical Interneuron Precursors in a Cell-Type-Specific Manner’ has just been published in Cerebral Cortex with Zsófia László as first author and Zsolt Lele as corresponding author. In this study, we describe the important role of N-cadherin, a cell adhesion protein in the migration and survival of cortical interneurons during the postmitotic stage of development. The results show that the effect of N-cadherin is cell-type-specific. Therefore, the removal of N-cadherin from the postmitotic neuroblasts leads to a change in the interneuron profile of the adult cortex due to the decrease in the number of GAD65-positive inhibitory interneurons. Well done Zsófi and Zsolt! For further reading see: https://academic.oup.com/cercor/article/30/3/1318/5538384 |
Our research takes a new direction and our lab story starts a new chapter02/01/2020 |
We are proud to report that our PI received the honor of becoming a Naus Family Endowed Chair in Addiction Sciences in the Department of Psychological and Brain Sciences at the Indiana University. Therefore, part of our team will soon join to an outstanding research community, a leading center for cannabinoid research in North America that is located in the wonderful college town of Bloomington. István’s new lab will be next to the laboratories of two Cannabinoid GRC chairs Ken Mackie and Andrea Hohmann, to the Past President of the International Cannabinoid Research Society Heather Bradshaw and other famous cannabinoid researchers such as Hui-Chen Lu and Alex Straiker. Besides strengthening our on-going collaborations in cannabinoid research, a major driving force for our team is to extend our methodical and conceptual expertise in human cognitive neuroscience. Because the Department of Psychological and Brain Sciences is ranked as a Top10 research location in cognitive psychology in the US, we will have a unique opportunity to learn from the best colleagues and combine our existing expertise in state-of-the-art nanoscale molecular, anatomical and electrophysiological techniques with novel mesoscale functional brain imaging and behavioral models. The newly formed „Addiction and Neuroplasticity laboratory” will primarily focus its research on substance use disorder-related pathological plasticity processes in both mice and humans. If you are interested in joining our Bloomington team please contact István: ikatona@iu.edu For further details see also: https://psych.indiana.edu/directory/faculty/katona-istvan.html |
A new article published in Science in collaboration with the lab of Ádám Dénes01/31/2020 |
As a result of a successful collaboration with Ádam Dénes and his lab at the Institute of Experimental Medicine, we are glad to report that our contribution appeared in the prestigious journal Science. This study elucidates a novel form of microglia-neuron communication that is essential for the monitoring and protecting neuronal functions by the microglia. The results identified a novel morpho-functional interaction site between the two cell types. Its formation is induced rapidly and requires the function of microglial purinoceptor P2Y12. The key lab members in this project from our team were Zsófia László and Zsolt Lele. For further reading see: https://science.sciencemag.org/content/367/6477/528.long |
Our new paper in Nature Neuroscience highlights that maternal cannabis abuse risks abnormal brain development in the embryo17/10/2019 |
We are delighted to announce that a novel study presenting some of our latest work has just been published in Nature Neuroscience. See details of the paper here.
The experimental findings of the new paper demonstrate that maternal THC exposure causes substantial molecular, cellular and synaptic changes in dopaminergic neurons of the ventral tegmental area in male offspring. The altered dopaminergic circuitry may represent a classic example of an endophenotype, because the preadolescent offspring seem to be normal at first look, but responds with abnormally elevated dopamine levels and aberrant behavioral patterns to THC exposure later in life. Importantly, the neurosteroid pregnenolone could rescue several aspects of the maladaptive alterations caused by maternal THC. These findings may contribute to a better understanding of the results of longitudinal clinical studies reporting that children of heavy cannabis-smoker mothers are at higher risk to develop neuropsychatric disorders. Because of the neurodevelopmental vulnerability, these results also argue against the current bad practice of proposing cannabis to pregnant women to alleviate morning sickness. This study emerged from our long-standing collaboration (supported by our joint NIDA grant) with the team of Miriam Melis from the University of Cagliari, Italy who led the consortium and the team of Joe Cheer from the University of Maryland, USA. We are also very grateful for Dr. Csaba Pongor from the Nikon Imaging Center at our institute for his help as a co-author in this project. |
Istvan Katona is organizer of the prestigious Gordon Research Conferences, Cannabinoid Function in the CNS 2019: Better Maps for Retrograde Transmission: Novel Molecular, Synaptic and Circuit Mechanisms
05/2019 |
This year’s scientific program is really exciting with a highly prestigious speaker line-up (please, refer to the attached flyer). The conference will take place at the Rey Don Jaime Grand Hotel in Castelldefels, Spain on the 21–26th July, 2019. For the detailed program and registration, please, check out the conference webpage here. |
Double success at the 34th National Student Competition26/04/2019 |
Congratulations to Krisztina Kelemen on winning II. Prize at the Physiology, Pathology Session of the 34th OTDK competition! Krisztina worked i
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New spring, new results18/03/2019 |
As the almond tree is blossoming again in the yard of our institute, several of our projects on the 9th floor are „flourishing”, too. We have really exciting new results in the pipeline ready to be published in the near future. Please, visit back soon, as exciting news are bound to come.
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Farewell to Ana Bernal Chico!02/2019 |
We wish her exciting new discoveries and lot of success! Everyone from the lab will definitely miss her a lot! |
Dennis Gabor Prize awarded to László Barna!13/12/2018 |
We are proudly congratulating our colleague, László on winning the Dennis Gabor Prize. This prestigious prize was founded in 1989 in honour of Dennis Gabor, inventor of holography. The prize is awarded every year to those Hungarian scientists who performed exemplary innovative scientific activity. László received the prize in recognition of his work in introducing the STORM super-resolution microscopy imaging to neuroscience research in Hungary and setting up and managing successfully the Nikon-KOKI Imaging Center with widely recognized activity in research and technical development. |
A scientific symposium “New approaches and applications of STORM super-resolution imaging in life sciences” organized by the PI of our lab, István Katona, was held at the Institute of Experimental Medicine 30/11/2018 |
The scientific conference aimed to bring together experts of Single Molecule Localization Microscopy in Hungary to discuss potential applications of the technologies PALM and STORM in neuroscience as well as in biophysics and biochemistry research. The speech of Prof. Tamás Freund, Director of the Institute of Experimental Medicine opened the conference. The scientific program included lectures by Prof. Klaudia Barabás (Center for Neuroscience, University of Pécs), Prof. Tibor Pankotai (Department of Biochemistry and Molecular Biology, University of Szeged), Prof. György Vámosi (Department of Biophysics and Cell Biology, University of Debrecen). On the part of the Institute of Experimental Medicine young researchers, Dr. Balázs Pósfai (Laboratory of Neuroimmunology) and Dr. Susanne Prokop (Laboratory of Molecular Neurobiology) introduced the latest results and developments in using super-resolution microscopy to study dynamic cellular actions and in developing the new innovative PharmacoSTORM method to label proteins with fluorescently tagged ligands for STORM imaging. The occasion for the conference was that Nikon’s latest Super Resolution System N-STORM 5.0 has been recently installed in the Nikon-KOKI Imaging Center of the Institute of Experimental Medicine. For this reason, we were honoured to receive special guests from Nikon Corporation and Nikon Instruments Europe. In his opening speech, Mr Takaharu Sasaoka, President of Nikon Instruments Europe B.V., acknowledged the long-standing successful collaboration between Nikon and the Institute of Experimental Medicine in research development. Dr. Kenta Imai, engineer from the Nikon Corporation, presented new features and future directions of development in super-resolution imaging and its applications. Dr. László Barna, Head of the Nikon-KOKI Imaging Center summarized experiences on managing the microscopy core facility that serves a large research community. The conference was closed by the ceremony of ribbon-cutting and a reception. |
The MNB lab at the SfN 2018 Meeting in San Diego11/2018 |
Our PI, István Katona was an invited speaker at the Symposium: „New observations in Neuroscience using superresolution microscopy” together with Michihiro Igarashi and Ed Boyden. He presented the combined patch-clamp and STORM imaging technique developed in our lab that enables the correlation of physiological, morphological and molecular parameters of the examined synapses of identified cell types. Four young students of the lab presented posters of their on-going projects followed by great interest of the participants of the meeting.
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Congratulations to Judit Glavinics!30/11/2018 |
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A new study on super-resolution microscopy has been published with the contribution of our laboratory!31/10/2018 |
As a recognition of our results achieved with STORM superresolution microscopy (SM) in the field of neuroscience, our PI, István Katona and László Barna, Head of the Imaging Center in our Institute have been invited to contribute to a review article in Journal of Neuroscience. The paper brings together experts of the different superresolution techniques to draw attention to the potential applications of these imaging techniques in neuroscience ![]() |
Congratulations to Vivien Miczán, Susanne Prokop and Judit Glavinics!02/09/2018 |
Our young colleagues received the prestigious state fellowship, UNKP, dedicated to the most talented students (undergraduate, graduate students and predoctoral fellows) who take part in scientific research programs.![]() |
The group had a great time at the 15th MNB lab retreat in the Bükk National Park!20/06/2018 |
This year the group returned to the Bükk Hills where the first MNB lab retreat was held back in 2003. The scenic location of Bánkút with its amazing beech forests provided an ideal setting for the 20 km hiking. On the way we saw wolf footprints and several species of orchids. Reaching the peak of Tar-kő we were rewarded by the breathtaking view of the Bükk Hills.![]()
The scientific program of the retreat was again really versatile with 14 exciting scientific lectures covering all the current projects of the team. The traditional „Hyper-active Prize” for the most active participant in the discussions was awarded to Anita Bernal for her useful comments. The „Hyper-attractive Prize” for the best quality presentation was awarded to Beni Barti. |
A new study in collaboration with Prof. Hanns Ulrich Zeilhofer at the University of Zurich has just been published in the Journal of Neuroscience!22/11/2017 |
The paper provides evidence that supraspinal CB1 cannabinoid receptors are essential for the analgesic effect of acetaminophen (aka paracetamol) against inflammatory pain. The use of various cell-type-specific CB1 deficient mouse lines and viral approaches together with microscopical investigations helped to unravel that especially those CB1 receptors, which are located in the rostral ventromedial medulla in the brainstem are among the most important molecular players in acetaminophen analgesia.
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Congratulations to Kata Kenesei on successfully defending her PhD thesis!09/10/2017 |
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Welcome back Vincent!09/10/2017 |
Vincent Paget-Blanc returns to our laboratory to![]() |
Welcome to Julia Leschik!10/04/2017 |
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Congratulations to Krisztina Kelemen!01/04/2017 |
Krisztina Kelemen won first prize at the Physiology, ![]() |
Ana Bernal Chico received a prestigious postdoctoral fellowship!01/02/2017 |
Congratulations to Ana who received a prestigious![]() |
A warm welcome to Claudia Sagheddu!03/01/2017 |
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We are pleased to welcome dr. Claudia Sagheddu from the University of Cagliari who is visiting our lab with a Short Term EMBO fellowship. She will stay for 3 months and will learn STORM super-resolution microscopy. |
A new study from the lab in terms of a fruitful collaboration with the teams of Pablo Castillo and Bryen Jordan at Albert Einstein College of Medicine in New York is just published in Neuron! 19/10/2016 |
The paper demonstrates that presynaptic protein synthesis is necessary for an endocannabinoid-mediated form of long-term depression. Moreover, the superior detection sensitivity of STORM imaging helped to provide the first direct microscopic evidence -to the best of our knowledge- that eukaryotic ribosomes are present in axon terminals in the adult mammalian brain.
See attached image and the full version. Some further details: It is well established that postsynaptic protein synthesis is critical for the consolidation of long-term synaptic plasticity. However, whether presynaptic local protein translation is involved remained highly debated within the field. In this study, Tommy Younts and Hannah Monday from the Castillo group have done meticulous paired patch-clamp recordings to show that a form of endocannabinoid-dependent long-term depression of inhibitory synapses in the hippocampal CA1 region requires protein synthesis, and can be blocked by the application of translation inhibitors in the presynaptic, but not in the postsynaptic cell. Their experiments also suggested that the site of translation is likely to be in distal axons, and not in the cell body. The presence of ribosomes in mature mammalian axon terminals has been a subject of controversy for decades. STORM super-resolution imaging available in our institute at the Nikon Center of Excellence for Neuronal Imaging offers unprecedented sensitivity in detecting immunofluorescence signal owing to the single-molecule sensitivity of the method. Barna Dudok in our lab has used STORM to demonstrate the presence of presynaptic ribosomes in hippocampal GABAergic axon terminals. Together, these results indicate that precisely regulated local protein synthesis allows the long-lasting modification of the molecular machinery for presynaptic neurotransmitter release at CB1-expressing inhibitory synapses. These findings also raise several exciting questions to be explored. Is presynaptic translation important for other forms of synaptic plasticity and does it play a role at other types of synapses? Furthermore, which mRNAs are specifically translated in axon terminals? To be continued… |
Barna Dudok in “Everone’s Academy” TV program04/10/2016 |
Barna was invited to give an educational lecture in the program Everyone’s Academy of the Hungarian channel M5TV. He introduced the endocannabinoid system and the effect of cannabis on neuronal activity in a colourful and entertaining way to the lay public. |
A warm welcome to Susanne Prokop!01/09/2016 |
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Goodbye to Steve Woodhams!31/08/2016 |
We will all certainly miss him a lot, not only his scientific expertise and helpful advices but also his cheerful and friendly personality and brilliant sense of humour. |
Farewell BBQ party to Steve and the first official MNB Lab Button Soccer Championship26/08/2016 |
To teach Steve how to play it – and to have fun – we held the first MNB Lab Button Soccer Championship. Istvan took the role of referee, as he would have had an unfair advantage in the chamionship having played this game A LOT in his childhood. Steve did a really good job in learning the game, as he ended up being the first Button Soccer Champion of the MNB lab. Congratulations, Steve! |
A warm welcome to Ágnes Varga!01/08/2016 |
Ágnes was very enthusiastic and got acquainted with a lot of techniques used in our lab. We wish her good luck with her studies! |
Barna and Barna in a scientific radio show talking about STORM!09/06/2016 |
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Master’s Thesis Defense of Miklós Zöldi
07/06/2016 |
Miki defended his Master’s thesis summa cum laude at the Eötvös Loránd University. The title of his thesis: The effect of radiation on endocannabinoid signaling system. Congratulations! |
Congratulations to István Katona!02/05/2016 |
We are pro![]() |
VividSTORM software available for download!
30/01/2016 |
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A study authored by 50% of the lab published in Nature Neuroscience!20/01/2015 |
Years after unpacking the new STORM microscope, we finally celebrate the publication of our paper in Nature Neuroscience. In this highly collaborative study – involving 11 members of the lab and the groups of Imre Kacskovics from Immunogenes, ltd., Ivan Soltesz from Irvine, Miriam Melis and Marco Pistis from Cagliari, Masahiko Watanabe from Sapporo, and even a mathematician, Máté Matolcsi from Budapest – we demonstrate the use of STORM super-resolution microscopy to study nanoscale molecular organization on individually labeled and identified neurons.![]() Using this technique, we show the unexpectedly homogeneous distribution of cannabinoid receptors on inhibitory axon terminals, speculate on the consequences of this on endocannabinoid signaling, and provide some shocking images to see what cannabis use does to your GPCRs. Please check out the full paper for details. |
New paper published8/07/2015 |
In our latest paper in The Journal of Neuroscience, with the labs of Ivan Soltesz and George Horvai, we reveal new mechanisms of tonic endocannabinoid signaling. Interestingly, tonic activity of CB1 cannabinoid receptors is determined by constitutive activity, instead of, as we originally expected, continuous activation by endocannabinoids. Moreover, this constitutive receptor activity is restricted to certain interneuron subtypes. To further complicate the picture,![]() |
Congratulations to Vivien Miczán!29/06/2015 |
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Our latest study is now accepted for publication26/03/2015 |
Following up on our earlier ventures on the Fragile X territory, we now published a new paper in Frontiers in Cellular Neuroscience. Mutation of the gene encoding FMRP causes Fragile X syndrome, a severe, monogenically inherited mental disability, also linked to autism. ![]() |
A new collaborative study published in Neuron03/05/2014 |
We are pleased to announce that our joint findings with the lab of Sachin Patel about the molecular architecture and function of synaptic endocannabinoid signaling in the central amygdala was published in Neuron last week. ![]() The central amygdala plays a fundamental role in the regulation of stress response and emotional learning. Notably, these two behavioral processes are strongly affected by cannabis use. The underlying neurobiological substrate for the cannabis effects were only partially understood. In addition, it was a long-standing question of how endocannabinoid signaling is involved in these mental processes. Using highly sensitive and specific antibodies against the major endocannabinoid-synthesizing enzyme, DGL-alpha (generated by Ken Mackie) and against CB1 receptors (developed by Masahiko Watanabe), Rita Nyilas in our lab could provide direct anatomical evidence that these key endocannabinoid signaling molecules are present at excitatory glutamatergic synapses in the central amygdala. Because previous CB1 antibodies did not reveal the presence of the receptor in this brain region, this was an unexpected finding, which we validated in CB1 knockout animals (see thumbnail). In accordance, Teniel Ramikie and her colleagues in the Patel lab obtained solid evidence for the existence of several phasic and tonic forms of synaptic plasticity at these synapses, which are mediated by endocannabinoid signaling. An especially intriguing finding of Teniel and Sachin is the strong muscarinic cholinergic regulation of these synaptic signaling processes. These findings pose several important questions of how the cholinergic tone and endocannabinoid signaling, both of which signals about the internal state of the animal, can interact with each other at the behavioral level and how these mechanisms can be therapeutically exploited. |
New paper from the MNB Lab01/02/2014 |
We are really proud to report that a project on the distribution of monoacylglycerol lipase (MGL) in the dorsal horn of the spinal cord could finally be successfully accomplished. The findings are published in the special PAIN issue of the European Journal of Neuroscience. The credit for this work should primarily go to Eszter Horváth and Steve Woodhams, and also to Masahiko Watanabe for kindly providing us a good antibody for MGL. This project has been running for a ![]() |
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A new baby lab member arrives!26/01/2014 |
Eszter Horváth has quite prolific weeks. Within just a few days her paper will appear online in the European Journal of Neuroscience. But now we are delighted to report something much more important: she gave birth to her third child, a beautiful girl called Lili Orbán. Warmest congratulations to Eszter and welcome Lili among us! We wish both of you good health, lots of fun to enjoy the milestones of “postnatal maturation” and lots of energy to run the family! |
Welcome to Marco Ledri!01/01/2014 |
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Merry Christmas and Happy New Year from the MNB LAB!24/12/2013 |
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Christmas party of the MNB LAB08/12/2013 |
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István Katona becomes elected member of the Academia Europaea15/10/2013 |
We are happy and proud to announce that our group leader, István Katona was elected to be the member of the Physiology and Medicine section of the Academia Europea. This honor recognizes his contributions to our understanding of endocannabinoid signaling in the brain. The Academia Europaea, the European Academy of Sciences is a pan-European organization of eminent scholars covering the full range of academic disciplines. We congratulate István for his achievements and wish him several more unexpected discoveries during his scientific carrier. |
Welcome to Emese Kovács!13/10/2013 |
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Autumn bike trip of the MNB LAB05/10/2013 |
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Welcome to Benjámin Barti!12/09/2013 |
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Gordon Research Conference on cannabinoid signaling in New England04-09/08/2013 |
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István Katona wins the Momentum Prize of the Hungarian Academy of Sciences01/07/2013 |
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Welcome to Vivien Miczán!01/07/2013 |
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Szilárd Szabó moves to the University of Freiburg, Germany as a postdoctoral researcher
27/06/2013 |
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Welcome to Daniel Ganszky!21/05/2013 |
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Many congratulations to Zsófia László!27/06/2013 |
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Spring Lab Retreat of the MNB LAB in Felsőtárkány05-07/04/2013 |
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Welcome to Ana Bernal Chico!14/01/2013 |
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Congratulations to Ashley Dorning!07/2012 |
Ashley is an avid Manchester United fan and her expertise in football became clear to all of us in this year’s betting competition for the EuroCup. Ashley correctly predicted the final results of 50% of the group matches and predicted two weeks before the start that Spain will win, Torres will be the top scorer and Ireland receives the highest number of goals. She came first out of the 11 participants. It was great fun to watch several games together in the nearby open-air pub, thanks a lot to Chris for organizing the game! |
Three new papers from the MNB Lab have just been accepted!07/2012 |
So we ran out of babies (temporarily), but we have received some really good news last week. Three studies involving the work of the MNB Lab were accepted in Nature Communications, The Journal of Neuroscience and in Journal of Physiology-London. More details are coming soon! Till then just check out the abstracts at the forthcoming FENS Forum in Barcelona. |
A new review on endocannabinoid signaling in Annual Review of Neuroscience!21/07/2012 |
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Congratulations to Balázs Pintér!06/2012 |
So, it is probably the time to recruit a nanny for the MNB lab! We were delighted to learn that the third girl and the fourth baby altogether for 2012 just arrived. Now the proud father is Balázs, and his cute little daughter is called Kata Virág Pintér. Mummy and Kata are healthy and happy, Daddy looks a little bit sleepy these days in the lab, just like his daughter! We wish the whole family a wonderful life together! |
One baby one paper!18/06/2012 |
So we keep the roll going! We were delighted to learn that a brand new study including the work of our lab member László Barna was just accepted in PLOS ONE. Laci used super-resolution microscopy to help the team of Balázs Gereben reveal that the active center of the type 3 deiodinase D3 enzyme is located on the outer surface of dense core vesicles in the axon of neurosecretory neurons. Kalló I, Mohácsik P, Vida B, Zeöld A, Bardóczi Z, Zavacki AM, Farkas E, Kádár A, Hrabovszky E, Arrojo E Drigo R, Dong L, Barna L, Palkovits M, Borsay BA, Herczeg L, Lechan RM, Bianco AC, Liposits Z, Fekete C, Gereben B (2012) A novel pathway regulates thyroid hormone availability in rat and human hypothalamic neurosecretory neurons. PLoS One. 7:e37860. PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/22719854 |
Congratulations to Eszter Szabadits!05/2012 |
Happy baby shower! We were happy to learn that Eszter gave birth to the third MNB Lab baby this year, she is called Luca Emese Cserép! Luca was a little bit impatient to see the outside world, but her biometric parameters are very good 2560 g and 49 cm. We wish Luca, Eszter and the whole family all the best! |
A new study from the MNB lab published in JCN!01/05/2012 |
Today, Journal of Comparative Neurology has published the study of our lab member Zsolt Lele describing the developmental and adult distribution of all type II cadherins in the hippocampus and the cortex. This work is the first detailed characterization of these crucial cadherins in cortical regions and it will serve as an important reference for those who study neuronal adhesion in cortical circuits. Lefkovics K, Mayer M, Bercsényi K, Szabó G and Lele Z (2012) Comparative analysis of type II classic cadherin mRNA distribution patterns in the developing and adult mouse somatosensory cortex and hippocampus suggests significant functional redundancy. Journal of Comparative Neurology 520:1387-1405. PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/22102170 |
Congratulations to Zsolt Lele!04/2012 |
It seems that our lab will have a prolific year in terms of both babies and scientific papers. Zsolt had to leave our lab retreat early and the next day his spouse delivered a beautiful little boy called Levente! Everyone is happy and relaxed now, and we wish Levente and the whole family a very peaceful childhood together! |
New equipment grant for super-resolution microscopy won by the PI of the MNB lab!23/04/2012 |
Prof. József Pálinkás, the President of the Hungarian Academy of Sciences officially announced today that our grant application was successful and we will receive the support to purchase a super-resolution microscope based on the STORM approach as well as an electron microscope with tomography. The grant amounts to 258 million HUF (860 000 Euro). The proposed project “Nanoscale architecture of the brain” will be accomplished in terms of the NANOBRAIN consortium which consists of a selected group of IEM HAS researchers led by István Katona, the PI of the MNB lab. Our efforts are integrated into our current international grants, because these new microscopes will greatly facilitate our experimental repertoire and open the possibility to study signaling pathways at the nanoscale level. |
2nd Spring MNB Lab Conference and Retreat in Tihany13-15/04/2012 |
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Welcome to Steve Woodhams!06/04/2012 |
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Another paper from the MNB lab!03/2012 |
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The MNB Lab in Southern California, Irvine02/2012 |
Three lab members were fortunate enough to escape the harsh European winter by partaking in an on-going collaboration with the group of Prof. Ivan Soltesz in the Department of Neurobiology and Anatomy, University of California, Irvine. The aim of our joint project with the Soltesz lab is to correlate endocannabinoid-dependent physiological parameters of synaptic activity with quantitative cell type-specific molecular profiling. We are using a combination of paired whole-cell patch-clamp recording with the STORM super-resolution approach to achieve this aim. The experiments were more than promising, so we are planning to continue to work together (and hope to get another chance to enjoy sunny Southern California). We also have a new team name ![]() |
Aniko Ludanyi moves to University College London as a postdoctoral researcher06/02/2012 |
Aniko has worked with us for more than 7 years and has greatly contributed to the MNB lab both scientifically and personally. Therefore, in a way, it is a sad moment for us that she leaves for London. On the other hand, we are fully aware that it is very important for Anikó’s scientific carrier to learn new methods and concepts in other laboratories during her postdoctoral stage. We are very proud that she was accepted into the world class laboratory of Prof. Antonella Riccio in the MRC Laboratory for Molecular Cell Biology at UCL, UK. We wish Anikó all the best and hope that she will make some great discoveries there! |
Congratulations to Rita Nyilas!31/01/2012 |
We were delighted to learn that Zsófia Varga, the first daughter of Rita Nyilas was born last night. Both the baby and Rita are healthy and happy. Her biometric parameters are 3 kg and 48 cm. As you can see below, Zsofi’s curiosity already captures the exciting external world and she listens to her mummy as every good kid should! We wish the whole family a healthy and long happy life together! |
Welcome to Ashley Dorning!10/01/2012 |
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Christmas Party in the MNB Lab18/12/2011 |
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Hungarian Television broadcasting from the MNB Lab06/12/2011 |
The Hungarian National Public Service Television Company (equivalent of the BBC and abbreviated as MTV or m1) recorded a 30 minute-long documentary about the life and work of the Laboratory of Molecular Neurobiology. It was a very funny day, we tried our best to behave as a mixture of serious scientists and Hollywood stars. The MTV crew did a really good job and illustrated the concepts of endocannabinoid signaling and the methods we use very well. The final documentary is found in the repository of MTV (not the one you would first think of) at the following link: http://videotar.mtv.hu/Videok/2011/12/06/15/Almok_mai_almodoi__Kalandozas_az_idegsejetek_kozott.aspx One needs Windows Media Player to watch the film. You can find additional images in our Lablife section. |
Welcome to Szilard Szabo!4/12/2011 |
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A new paper from the MNB lab!11/2011 |
We are happy to report that EMBO Fellow Chris Henstridge has just had an invited review published in Molecular Endocrinology. This review summarizes new information on GPR55, an interesting but as yet orphan GPCR, which in some paradigms can be activated by distinct cannabinoid ligands. Henstridge CM, Balenga NA, Kargl J, Andradas C, Brown AJ, Irving A, Sanchez C, Waldhoer M (2011) Minireview: recent developments in the physiology and pathology of the lysophosphatidylinositol-sensitive receptor GPR55. Molecular Endocrinology 25:1835-48. PubMed link: http://www.ncbi.nlm.nih.gov/pubmed/21964594 |
Annual MNB Lab autumn biking trip9/10/2011 |
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MNB Lab at the FENS Featured Regional Meeting in Ljubljana22-25/09/2011 |
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Welcome to Eszter Szabadits!01/09/2011 |
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Welcome to Eszter Székely!01/08/2011 |
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Chris Henstridge delivers lecture at the British Neuroscience Association Meeting17-20/04/2011 |
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PhD defense of Anikó Ludányi05/04/2011 |
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1st Spring MNB Lab Conference and Retreat at Mátraháza2-3/04/2011 |
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